If you are looking for detailed information on lateral flow test cassettes, here is another post for you.
Finding information on the internet is not that difficult but finding the correct information on lateral flow test cassettes is not that easy! Confused?
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Lateral Flow Test Cassette: All about it
What is Lateral Flow Test Cassette, and how does it work? It’s a basic diagnostic device that confirms the presence or absence of a target analyte, such as infections or biomarkers in humans or animals, or pollutants in water, food, or animal feed.
A control line, as well as one or more target or test lines, are usually included in LFDs to ensure that the test is running properly. They are built with intuitive user conventions in mind and require very little training to operate.
They can be qualitative and read visually, or they can be quantitative and give data when used in conjunction with reader technology.
To obtain findings, Lateral Flow Test Cassette design employs immunoassay technology, which involves a nitrocellulose membrane, colored nanoparticles (or labels), and usually antibodies.
When a sample is supplied to the test device, it will flow through the conjugate pad, into the nitrocellulose membrane, and finally onto the absorbent pad.
In human health, lateral flow tests are commonly used for point-of-care testing. They can be carried out by a healthcare professional or by the patient in a variety of locations, including the laboratory, clinic, or the patient’s own home.
In the medical diagnostic sector, rigorous regulatory standards must be followed for all goods produced and manufactured and rapid test cassette manufacturers will help you with this.
The following bullet points show how the lateral flow test cassette assembly works.
- The sample pad is the initial stage of the absorption process as told by rapid test cassette manufacturer, and it may incorporate a filter to guarantee that the sample flow is precise and regulated.
- The sample will be sent to the conjugation pad, which has the conjugated labels and antibodies. If the target is present, the immobilized conjugated antibodies and labels will bind to it and move through the test.
- The binding reagents on the nitrocellulose membrane will bind to the target at the test line as the sample travels along the device. A colored line will appear on the lateral flow test cassette, with the density varying based on the amount of target present.
- To ascertain target concentration, some targets may need quantification. When a quick test is coupled with a reader, quantifiable data can be obtained.
- The sample will be absorbed by the absorbent pad after passing through the nitrocellulose membrane. The surplus sample will be absorbed by the absorbent pad. The absorbent pad’s specifications will influence the amount of sample that may be included in the test.
This is all about the working and functionality of lateral flow test cassette. Happy learning.